Ph.D. Topics

CRISPR-mediated genome editing in Brassicaceae

Supervisor: Veronika Jedličková

Annotation:  

The CRISPR/Cas system is one of the most promising methods for genome editing in various organisms. In our laboratory, we developed a method for CRISPR-based genome editing in oilseed rape (Brassica napus) and Arabidopsis thaliana using hairy root cultures to study specific genes and their regulatory sequences. The PhD candidate will use molecular biology approaches to assess the functionality and efficiency of various CRISPR editing constructs, transformation methods (stable versus transient), and regeneration protocols to develop transgene-free, genome-edited plant lines in various Brassicaceae species.

Funding of the PhD candidate: 

The topic is funded by the OP JAK TANGENC project.

PhD programme:

Genomics and Proteomics, Faculty of Science, MUNI

Requirements for candidate:

The candidate should have experience with molecular biology techniques (DNA/RNA isolation, cloning, RT-qPCR), gene reporter assays and microscopy. Additionally, the candidate should have some knowledge of bioinformatics.

Recommended literature: 

Jedličková, V. et al. (2022) Hairy root transformation system as a tool for CRISPR/Cas9-directed genome editing in oilseed rape (Brassica napus). Front Plant Sci.13: 919290. doi: 10.3389/fpls.2022.919290

Zhu, Y. et al. (2024) Plant hairy roots: Induction, applications, limitations and prospects. Industrial Crops and Products 219: 119104. doi: 10.1016/j.indcrop.2024.119104.

Cardi, T. et al. (2023) CRISPR/Cas-mediated plant genome editing: outstanding challenges a decade after implementation. Trends in Plant Science 28: 1144 – 1165. doi: 10.1016/j.tplants.2023.05.012

Jedličková, V. et al. (2024) Injection-based hairy root induction and plant regeneration techniques in Brassicaceae. Plant Methods 20: 29. doi: 10.1186/s13007-024-01150-1

Application:

PhD Candidate on CRISPR-based genome editing in plants